Understanding PCR

1. Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction

  • List common manufacturers of PCR reagents

1.1 Introduction

  • amplify fragments of DNA <- ใช้ PCR

  • molecular technique -> genetic profiling , detection , gene expression , genetic modification

  • ซื้อ Polymerase ได้ที่ไหน ?

1.2 Basic Laboratory preparation

  • ต้องสะอาด และไม่ contaminateจาก enviroment , หรือ poor handling of reagents

  • หรือ PCR inhibitor จาก consequence DNA preparation

  • โต๊ะ ต้องสะอาด , 70% alcohol

  • pipettes สะอาด, calibrated -> แน่ใจว่า precision

  • PCR reagents - kept on ice

  • label reagent ที่ใช้

  • Titrating template material / preparig replicate reaction

  • เตรียม master mix ingredient

  • planning PCR "on paper" -> design primers, reaction parameters


PCR workbench
PCR workbench

PCR Recipe List , Hints, and Tips


2. Designing and Ordering Your Polymerase Chain Reaction Primers


Reference

  1. OPDF Understanding PCR

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